SRU Biosystems - BIND Biosensors >> Cartridge Biosensors

BIND Biosensors >> Cartridge Biosensors

The heart of SRU’s BIND® technology is the novel optical properties of the BIND Biosensors. Each well of a BIND Biosensor incorporates a proprietary optical grating which reflects a narrow range of wavelengths (Peak Wavelength Value or PWV) upon illumination with broadband light. The PWV shifts in response to changes in mass or cell attachment in proximity of the biosensor surface. BIND 16-well cartridge biosensors have optical grating identical to that of BIND plate-based biosensors and thus provide the same superior performance and application versatility in a lower throughput system.

Cartridge Biosensor Features

16-well Format
Compatible with Biochemical & Cell-based Assays
Enable Cost Effective, Label-free Assays
Identical Optical Grating as Plate Biosensors Simplifying Migration to Higher Throughput Formats
Unmatched Quality for Robust, Reliable Assay Results

Cellular Application

Common cellular applications include GPCR activation, ion channel assays and cell adhesion assays. BIND’s label-free nature and its high level of sensitivity provide an extremely flexible cell-based assay system which can be used with both endogenous and recombinant receptor systems and is compatible with primary cells and adherent and suspension cell lines.

Agonist Titration (Carbachol)

Antagonist Titration (Atropine)

FIGURE 1: Agonist and antagonist dose response of endogenous muscarinic receptor in HEK cells. 2,500 HEK cells were added to collagen coated 16-well Biosensor Cartridges. Cells were activated with various concentration of carbochol (agonist) or incubated in the presence of 20 µM carbachol and various concentrations of atropine (known antagonist). Cell responses were measured following a 30 minute incubation using the BIND Cartridge Reader.

Biochemical Binding Assay

Commonly run BIND biochemical applications include small molecule binding, fragment-based screening, identification of promiscuous or aggregating compounds, protein-protein binding, enzyme assays and antibody characterization. Cartridge biosensors can be coated with a variety of biomolecules such as peptides, proteins, antibodies, biotinylated molecules and nucleic acids. Figure 2 depicts the results of a small molecule binding assay (data courtesy of JHU). A known small molecule inhibitor was assayed for binding to a bacterial protease. Does-dependent binding of the inhibitor was detected with very low assay %CVs.

Binding Assay - Small Molecule

Figure 2 - 16-well BIND cartridge biosensors were coated with a protease, followed by incubation with various concentrations of a small molecule inhibitor. Peak wavelengths (PWV) of samples were detected on the BIND Cartridge Reader. The results are shown for an N=4. Rmax was 88 pm and the %CV was <6.

Guaranteed Quality

SRU Biosystems rigorously tests each well of every cartridge prior to shipping to ensure a high level of Biosensor quality and superior assay performance. SRU tests a variety of parameters including uniformity, lot-to-lot consistency, shelf life and integrity of surface coatings.

Ordering Information

BIND Cartridge Biosensors
BIND cartridge biosensors can be used for both cell-based and biochemical applications and are constructed with the same optical grating as the plate-based BIND Biosensors. Cartridges have a 100 μl maximum working volume.

Product #	Description	Cell-based Assays	Biochemical Assays
TiO-CR-C	Bare TiO2 16-well cartridge w/clear finish, O2 plasma treated	x
GA3-CR-C	High density aldehyde 16-well cartridge w/clear finish		x
SA1-CR-C	Streptavidin coated 16-well cartridge w/clear finish		x
CA2-CR-C	Cell attachment matrix, 16-well cartridge w/clear finish	x