SRU Biosystems - BIND Reader >> PROFILER

BIND Readers >> PROFILER

The BIND® PROFILER is a small footprint, high throughput plate reader for the label-free measurement of a variety of biomolecular interactions. The PROFILER was designed with flexibility in mind to enable researchers to seamlessly move from target discovery and assay development to full screening and compound profiling. It is ideal for GPCR and ion channel assays, including endogenous receptor systems and assessment of primary cells, as well as for biochemical assays such as protein:protein binding, fragment-based and small molecule screening assays.

PROFILER Features

96-, 384- and low volume 384-well read capabilities
Target discovery to compound profiling & screening using a single instrument
Kinetic & endpoint measurements produce information-rich data
High speed label-free detection times using TURBO read mode
Real-time data acquisition & viewing

Profiling to Screening with a Single Instrument

A. Multi-component Binding Assay: Nuclear Recp.

B. Nuclear Receptor High Throughput Screen

Figure 2 - Biotinylated cofactor peptide was immobilized on Streptavidin-coated BIND Biosensors (SA1). Biosensors were placed on the reader and measurements taken throughout the entire assay. A). Test compounds were added first, followed by the addition of the nuclear receptor. B). The 10K compound screen was performed using the same assay protocol. Identified hits were confirmed for concentration-dependent activity (data not shown). Data courtesy of GSK.

The PROFILER’s kinetic read mode allows for visualization of individual binding events over time. This information can provide insights into mechanisms of action such as competitive vs. non-competitive binding and cofactor requirements. Figure 2A displays data from a nuclear receptor (NR) binding assay in which small molecular weight compounds are assessed for agonist and antagonist activities. A peptide known to bind the NR was immobilized onto BIND Biosensors and then compounds added. The initial lack of response indicates that none of the compounds directly bound the peptide. Three levels of binding were observed upon subsequent addition of the NR. Those wells containing agonists showed the highest binding activity, while the wells containing antagonists had responses similar to control wells which were not coated with peptide. As expected, those wells with no compounds added showed moderate, but not maximal, NR binding.

This assay was used to screen an orphan NR against 10,000 small molecule compounds. 32 specific, titratable agonists were identified, several with super-stoichiometric binding (fig. 2B).

Unmatched Detection Times Using SRU’s
TURBO Read Mode

The PROFILER is available with an optional TURBO read mode that decreases read times by up to 75%. A 96- well BIND Biosensor can be read in <10 seconds and a 384-well Biosensor in <20 seconds. Quick read times allow for shorter interval kinetic readings. The optional TURBO read mode can be installed at the time of purchase or is available as an in-field upgrade.

SRU’s EMS Software: Powerful User-Interface

All BIND Readers are supplied with SRU’s Experiment Management System (EMS) software. EMS provides complete control of assay parameters through an easy-to-use, intuitive interface and allows for visualization, export and secure storage of all data. Data can be exported from EMS using a custom export wizard in several file formats for maximum flexibility.

EMS User Interface

EMS Data Export Wizard

PROFILER Specifications

Compatible plates:	All 96-, 384-, and 384LV-well BIND® Biosensor microplates
Read Time:	96-well in 30 seconds, 384-well in 1 minute
TURBO Read Times:	96-well in 8 seconds, 384-well in 15 seconds
Operating Temperature Range:	4°C to 37°C
Dimensions:	15.5 in. (W) x 17.6 (D) x 14 in. (H)
Weight:	68 lbs.
Computer:	Computer and monitor included