BIND® Reader Applications
- Analysis of functional cell response in live cells expressing GPCRs, RTKs and ion channels
- Robust and reliable measurement of endogenous cell targets
- Compatible with adherent and suspension cell types
- Information-rich data allows clustering of compound activity based on pathway mechanism
- Affinity screening of small molecule and fragment libraries to protein targets
- Differentiate specific target binding from high stoichiometry and aggregation artifacts
- Compatible with protein-protein interaction and other difficult to address targets
- High throughput affinity ranking at the post fusion stage in HAT media with fewer false positives and false negatives
- Hybridoma screening, functional cell binding and epitope analysis applications
- Compatibility with complex media enables assays under native conditions
Drug Discovery Applications on the BIND Reader:
BIND® from SRU Biosystems is a universal assay system that enables label-free detection of drug-target interactions. The system is comprised of the BIND® Reader and 96-, 384- or 1536-well microplate BIND® Biosensors. The BIND system takes advantage of a novel optical effect to provide very sensitive measurements of changes in binding or adherence in the proximity of the biosensor surface.
Both biochemical and cell-based assays are easy with BIND. For example, when an antibody is immobilized on the biosensor surface, antigen binding can be detected without the use of a label. Each addition of an assay component to a well can be observed in real time to monitor both specific and nonspecific interactions, enhancing both assay development and screening quality control.