BIND® Reader Applications

Cell based assays

  • Analysis of functional cell response in live cells expressing GPCRs, RTKs and ion channels
  • Robust and reliable measurement of endogenous cell targets
  • Compatible with adherent and suspension cell types
  • Information-rich data allows clustering of compound activity based on pathway mechanism
Direct binding small molecule assays
  • Affinity screening of small molecule and fragment libraries to protein targets
  • Differentiate specific target binding from high stoichiometry and aggregation artifacts
  • Compatible with protein-protein interaction and other difficult to address targets
Antibody screening
  • High throughput affinity ranking at the post fusion stage in HAT media with fewer false positives and false negatives
  • Hybridoma screening, functional cell binding and epitope analysis applications
  • Compatibility with complex media enables assays under native conditions

Drug Discovery Applications on the BIND Reader:

BIND® from SRU Biosystems is a universal assay system that enables label-free detection of drug-target interactions. The system is comprised of the BIND® Reader and 96-, 384- or 1536-well microplate BIND® Biosensors. The BIND system takes advantage of a novel optical effect to provide very sensitive measurements of changes in binding or adherence in the proximity of the biosensor surface.

Both biochemical and cell-based assays are easy with BIND. For example, when an antibody is immobilized on the biosensor surface, antigen binding can be detected without the use of a label. Each addition of an assay component to a well can be observed in real time to monitor both specific and nonspecific interactions, enhancing both assay development and screening quality control.