Cell based assays - Universal Platform


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Use of suspension cells on BIND : THP-1 cell activation with chemokines
THP-1 cells were seeded into a 384-well BIND Biosensor coated with a proprietary
suspension cell binding surface. After 2 hour starvation, chemokine mediated
THP-1cell response was monitored over time. Prior to injection of chemokine a
stable baseline is obtained. Kinetic profiles of rapid cell response is possible
with BIND. MCP maximum response peaks in 10 minutes, then rapidly declines.
MIP-1α and Rantes also reach maximum response rapidly but then remain stable
over 60 minutes.
BIND label-free cell-based assays are compatible with most cell types including adherent, suspension, primary and recombinant. SRU Biosystems has developed proprietary surfaces and protocols for cell-based applications. The use of cells expressing the endogenous pathway target in your screening assay provides a physiologically relevant assay for selection of active compounds with desired selectivity. An assay developed on the BIND Reader in 96-well biosensors moves easily to 384 and 1536-well formats for screening applications.
  • Measurement of recombinant or endogenous targets
  • Compatible with primary cells
  • Use of adherent and suspension cell types
  • Kinetic measurement of rapid cell responses in HTS mode
  • Low cell numbers per assay
  • One platform designed to meet customer needs from assay development to screening


Activation of an endogenous target, such as a receptor
tyrosine kinase, produces a characteristic time dependent
response for each cell type (A431, HEK or CHO) on the
BIND Reader, highlighting the important differences in
pathway coupling in different cell backgrounds. Assay
conditions: 20,000 cells/well with endogenous EGFr
expression, 384-well BIND biosensor, 100nM EGF, flat
lines are no EGF, typical std errors <10%
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