Technology
BIND Technology
BIND® is a label-free assay technology that enables detection of a variety of biomolecular interactions including biochemical and cell-based readouts. BIND uses a novel optical effect to provide highly sensitive measurements of changes in binding or adherence in the proximity of the biosensor surface. Biosensors are produced with a proprietary nanostructured optical grating that is incorporated within each well of industry standard 96-, 384- and 1536-well microplates or 16-well cartridges. The optical grating reflects only a narrow range of wavelengths of light upon illumination with broadband light (“Peak Wavelength Value” or “PWV”). When a biomolecule or cell binds to the biosensor surface, this reflected wavelength increases (Figure 1). Real time binding can be detected by measuring PWV over time.
Wavelength Principals
 Figure 1 – As biomolecules or cells attach to the Biosensor surface the peak wavelength (PWV) of reflected light increases. The change in the PWV is proportional to the change in binding.
BIND Technology Features
  • Label-free, Homogeneous Assay Detection
  • Low to Ultra-High Throughput Capabilities
  • Universal Technology for Cells, Proteins and Small Molecules
  • Efficient Screening & Profiling with Decreased False Positives/False Negatives
  • Simplified Assay Development & Optimization
  • Cost Effective, Versatile Assay Platform

Cell-based Applications

When cells interact with the biosensor surface the reflected wavelength increases (Figure 2). Cells can be challenged with ligands/agonists/antagonists and changes in cell attachment to the biosensor attachment assessed. Decreases (negative change in PWV) and increases (positive change in PWV) in cell attachment can be detected using kinetic and endpoint read modes. Kinetic measurements allow for real time, information-rich data collection, while endpoint measurements allow for maximum assay throughput.
Cell Based Measurement		 Wavelength Principals Figure 2 - The principles of BIND applied to measuring cellular interactions with the biosensor surface. Changes in PWV can be either positive or negative reflecting tighter or looser levels of cell attachment. The tighter the association with the biosensor surface, the greater the PWV.

Biochemical Applications

A variety of biomolecules can be coated on SRU BIND Biosensors including peptides, proteins, antibodies, biotinylated molecules and nucleic acids. Upon interaction with binding partners, an increase in PWV is detected which is proportional to the change in mass (Figure 3). Binding of additional molecules in a multi-component binding assay causes further increase in the PWV. A variety of biochemical applications can be performed including small molecule binding, fragment-based screening, identification of promiscuous or aggregating compounds, protein-protein binding, enzyme assays and antibody characterization. BIND is a powerful development tool that lets you qualify and quantify binding at every step of your experiment.
Biochemical Measurements		 Wavelength Principals Figure 3 - The principles of BIND applied to measuring binding events. Increases in mass within proximity of the biosensor surface cause an increase in PWV. The change in PWVs is directly proportional to the change in mass.