BIND Biosensors >> Plate-based Biosensors
The heart of SRU’s BIND® technology is the novel optical properties of the BIND Biosensors. Each well of a BIND Biosensor incorporates a proprietary optical grating which reflects a narrow range of wavelengths (Peak Wavelength Value or PWV) upon illumination with broadband light. The PWV shifts in response to changes in mass or binding in proximity of the biosensor surface. SRU’s microplate-based BIND Biosensors are available in 96-, 384-, 384lv- and 1536-well formats with a variety of surface coatings to accommodate a breadth of throughput and application needs. All biosensors are biologically inert and highly resistant to organic solvents including DMSO.
Biosensor Plate Features
- 96- , 384-, 384lv- & 1536-well Biosensors
- Variety of Surfaces Optimized for Biochemical and Cell-based Assays
- Unmatched Quality for Robust, Reliable Assay Results
- Compatible with All BIND Plate-based Readers
- Conform to SBS Standards
Biosensor Applications
SRU offers a number of optimized surfaces for cellular and biochemical assays including bare TiO2, high and low density aldehyde surfaces for covalent linkage, streptavidin-coated for biotin capture and our proprietary CA2 cell matrix surface to promote cell attachment. CA2 biosensors are optimized for improved performance in cellular applications with adherent and suspension cells as well as primary cells.Cellular Applications
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Biochemical Applications
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Cell-based Assays
SRU Biosensors are compatible with both adherent and suspension cell types. Our proprietary CA2 surface promotes cell attachment and is recommended for use with both suspension and adherent cells. Bare TiO2 plates can be coated with your protein/biomolecule of choice. Figure 1 summarizes the results of a study examining two different GPCR overexpressing cell lines (Muscarinic M4 and M5 cells) challenged with three agonists using BIND 1536-well Biosensors. Biosensors were treated with four different surface coatings. Figure 1A depicts the results of a 10 point dose response Acetylcholine challenge. Figure 1B displays the results for all three agonists and control wells using a heat map format.
GCPR Assay
A. Acetylcholine Challenge of M4 Overexpressing Cells
A. Acetylcholine Challenge of M4 Overexpressing Cells
B. Heat Map Representation of Agonist Challenge of M4 and M5 Cells
Figure 1 - Chem-1 cells overexpressing either the M4 or M5 Muscarinic Receptor (cells courtesy of Millipore) were plated on a 1536-well BIND Biosensor with 4 different surface coatings. Cells were challenged with various concentrations of Acetylcholine, Carbanoylcholine, Pilocarpine or assay buffer alone. Cells were incubated with agonist for 30 minutes and the peak wavelength measured (PWV) on a BIND SCREENER. A). Acetylcholine dose response curve of M4 cells and the calculated EC50; B). Results for both M4 and M5 overexpressing cells on all surfaces viewed using the heat map format within the BIND reader EMS software.
Biochemical Assays
SRU Biosystems offers a number of optimized surfaces for immobilization of proteins, peptides, small molecules and nucleic acids. The BIND Biosensors commonly used for biochemical applications are bare TiO2 plates and aldehyde-coated plates. GA1 and GA3 biosensors are coated with pre-activated aldehyde surfaces, at a low and high densities respectively, to provide stable immobilization of targets via primary amine groups. In addition, SRU offers streptavidin-coated plates for use with biotinylated assay components. The results of a kinase binding assay are summarized in Figure 2. Figure 2A shows the binding of AMP Kinase to the biosensor surface over time versus the shift in PWV. The binding assay using the AMPK-coated biosensors successfully demonstrated the binding of the 130kDa AMP Kinase to AMP (365Da) despite the small molecular weight of AMP. Binding was specific, as no significant binding of GMP to the AMP Kinase was detected (data not shown). The 1:1 stoichiometry of AMP:AMPK binding was confirmed through the following equation: (∆PWV for AMPK biosensor binding/∆PWV of saturating amounts of AMP to AMPK-coated biosensors)*(molecular weight of AMPK/molecular weight of AMP). (0.026/8.4)*(130,000/365) = 1.1mol/mol.
Kinase Binding Assay
A. AMPK Immobilization
A. AMPK Immobilization
B. AMP Binding
Figure 2 - 384-well BIND biosensor plates were coated with AMP Kinase. Various concentrations of AMP were added to AMPK-coated and uncoated (Ref.) biosensors and PWV measured on a BIND reader. A). The time course for binding of AMP Kinase to BIND Biosensors; B). Binding curve for AMP on coated and uncoated biosensors.
Guaranteed Quality
SRU Biosystems rigorously tests each well of every microplate prior to shipping to ensure a high level of Biosensor quality and superior assay performance. SRU tests a variety of parameters including uniformity, lot-to-lot consistency, shelf life and integrity of surface coatings.
Ordering Information
Plate-based BIND Biosensors are provided with lids and are available in 96-, 384-, low volume 384- and 1536-well formats. Biosensor microplates have the following maximum volumes: 96-well (300 μl); 384-well (70 μl); low volume 384-well (28 μl); 1536-well (5 μl). Biosensors are compatible with automated liquid handling and conform to SBS standards. Custom coating of plates is available.
| Product # | Description | Cell-based Assays |
Biochemical Assays |
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| TiO-96-M | Bare TiO2 96-well plate | x |
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| TiO-96-CM | Bare TiO2 96-well plate, O plasma treated | x |
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| TiO-384-M | Bare TiO2 384-well plate | x |
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| TiO-384-CM | Bare TiO2 384-well plate, O plasma treated | x |
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| TiO-384LV-M | Bare TiO2 low volume, 384-well plate | x |
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| TiO-384LV-CM | Bare TiO2 low volume 384-well plate, O2 plasma treated | x |
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| TiO-1536-M | Bare 1536-well plate | x |
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| TiO-1536-CM | Bare TiO2 1536-well plate, O plasma treated | x |
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| GA1-96-M | Low density aldehyde 96-well plate | x |
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| GA1-384-M | Low density aldehyde 384-well plate | x |
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| GA1-384LV-M | Low density aldehyde low volume, 384-well plate | x |
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| GA1-1536-M | Low density aldehyde 1536-well plate | x |
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| GA3-96-M | High density aldehyde 96-well plate | x |
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| GA3-384-M | High density aldehyde 384-well plate | x |
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| GA3-384LV-M | High density aldehyde low volume, 384-well plate | x |
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| CA2-96-M | Cell attachment matrix, 96-well plate | x |
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| CA2-384-M | Cell attachment matrix, 384-well plate | x |
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| CA2-384LV-M | Cell attachment matrix, low volume 384-well plate | x |
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| CA2-1536-M | Cell attachment matrix, 1536-well plate | x |
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| SA1-96-M | Streptavidin coated, 96-well plate | x |
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| SA1-384-M | Streptavidin coated 384-well plate | x |
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| SA1-384LV-M | Streptavidin coated low volume, 384-well plate | x |
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